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Anti Tumor Effects of Conjugated Gold Nanorods with Interferon Gamma and Methionine

Masoud Ghorbani, Fatemeh Salehi Sarvestani

Abstract


Aim (Background): Hyperthermia or thermotherapy is a type of cancer treatment in which body tissue is exposed to high temperatures up to 45 ° C, usually with minimal injury to normal tissue. In the present study hyperthermia and gold nanoparticles were recruited to faster heat transmission and distribution to cells using near-inferared (NIR) laser light.

Materials and Methods: Ten nanometer gold nanorods were conjugated with interferon gamma (IFN-g) and methionine using diethylene triamine pentaacetic acid (DTPA) as a linker. The complex was then incubated with human breast adenocarcinoma cell line (MCF-7) at different concentration followed by irradiation with NIR laser beam. The cells viability were then evaluated using MTT assy.

Results: The average percentage of cell survival rate was approximately 85 percent at 0.3μg/ml of both conjugated and un-conjugated gold nanorods. The results of MTT assay showed that the 0.64 μg/mL concentration of gold nanoparticles complex was toxic on tumor cells (P < 0.05). The cell survival rate decreased to 77% in the presence of 0.30 μg/mL concentration of conjugated gold nanorods with IFN-g complex after irradiation with laser beams and hyperthermia.

Conclusion: The size and concentration of gold nanorods was not cytotoxic. Low concentration of gold nanorods at 0.3μg/ml did not cause a significant toxicity effect on MCF-7 cells, but the conjugated complex including gold nanorods and IFN-g increased the cell death rate after irradiation with Laser beam. This could be more due to the release of IFN-g and its toxicity effects on cells rather than hyperthermia generated by irradiation.


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